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rabbit polyclonal antibody against phosphorylated insulin receptor β subunit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody against phosphorylated insulin receptor β subunit
    Rabbit Polyclonal Antibody Against Phosphorylated Insulin Receptor β Subunit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+phosphorylated+insulin+receptor+%CE%B2+subunit/rabbit+polyclonal+antibody+against+phosphorylated+insulin+receptor+%CE%B2+subunit/10__1042_slash_bj20140155-59-37-48
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody against phosphorylated insulin receptor β subunit - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: GDF15 regulates Kv2.1-mediated outward K+ current through the Akt/mTOR signalling pathway in rat cerebellar granule cells
    Article Snippet: The proteins were separated by SDS/PAGE (10% gel) and were then transferred on to a PVDF membranes (Millipore).

    Membrane:

    Article Title: GDF15 regulates Kv2.1-mediated outward K + current through the Akt/mTOR signalling pathway in rat cerebellar granule cells
    Article Snippet: The proteins were separated by SDS/PAGE (10% gel) and were then transferred on to a PVDF membranes (Millipore). .. The membrane was blocked with 10% (w/v) non-fat dried skimmed milk powder and incubated at 4°C overnight with the following primary antibodies: mouse monoclonal antibody against Kv2.1 or Kv2.2 (1:1000 dilution; UC Davis, Davis, CA, U.S.A.), rabbit polyclonal antibody against phosphorylated insulin receptor β subunit (1:1000 dilution; Cell Signaling Technology), rabbit monoclonal antibody against phosphorylated ERK1/2 or total ERK1/2 (1:1000 dilution; Cell Signaling Technology), rabbit monoclonal antibody against phosphorylated Akt (1:2000 dilution; Cell Signaling Technology), rabbit polyclonal antibody against phosphorylated mTOR (mammalian target of rapamycin; 1:1000 dilution; Cell Signaling Technology), rabbit polyclonal antibody against TGFβR2 (TGFβ receptor 2; 1:500 dilution; Millipore), mouse monoclonal antibody against phosphorylated tyrosine (1:2000 dilution; Cell Signaling Technology), or mouse monoclonal antibody against GAPDH (glyceraldehyde-3-phosphate dehydrogenase; 1:10000 dilution; KangChen Bio-Tech). .. After extensive washing in TBST (TBS with 0.03% Tween 20), the membrane was incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:10000 dilution; KangChen Bio-Tech) for 2 h at room temperature.

    Article Title: Neuritin Activates Insulin Receptor Pathway to Up-regulate Kv4.2-mediated Transient Outward K + Current in Rat Cerebellar Granule Neurons
    Article Snippet: The proteins were separated on a 10% SDS-polyacrylamide gel, transferred to polyvinylidene difluoride membranes (Millipore). .. The membrane was blocked with 10% nonfat milk and incubated at 4 °C overnight with mouse monoclonal antibody against Kv4.2, Kv4.3, Kv1.1 (1:2000, catalogue numbers 75-016, 75-017, and 75-007; University of California, Davis), or rabbit polyclonal antibody against the phosphorylated insulin receptor β subunit (1:1000, catalogue number 3024; Cell Signaling Technology), or rabbit monoclonal antibody against phosphorylated ERK1/2 or total ERK1/2 (1:1000, catalogue numbers 4370 and 4695; Cell Signaling Technology), or rabbit monoclonal antibody against phosphorylated Akt (1:2000, catalogue number 4060; Cell Signaling Technology), or rabbit polyclonal antibody against phosphorylated mTOR (1:1000, catalogue number 2971; Cell Signaling Technology), or rabbit polyclonal antibody against phosphorylated IRS-1 (1:500, catalogue number 3070; Cell Signaling Technology), or mouse monoclonal antibody against GAPDH (1:10,000, catalogue number KC-5G4; KangChen Bio-Tech, China). .. After extensively washing in TBST, the membrane was incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:10,000, catalogue numbers KC-MM-035 and KC-RB-035; KangChen Bio-Tech) for 2 h at room temperature.

    Incubation:

    Article Title: GDF15 regulates Kv2.1-mediated outward K + current through the Akt/mTOR signalling pathway in rat cerebellar granule cells
    Article Snippet: The proteins were separated by SDS/PAGE (10% gel) and were then transferred on to a PVDF membranes (Millipore). .. The membrane was blocked with 10% (w/v) non-fat dried skimmed milk powder and incubated at 4°C overnight with the following primary antibodies: mouse monoclonal antibody against Kv2.1 or Kv2.2 (1:1000 dilution; UC Davis, Davis, CA, U.S.A.), rabbit polyclonal antibody against phosphorylated insulin receptor β subunit (1:1000 dilution; Cell Signaling Technology), rabbit monoclonal antibody against phosphorylated ERK1/2 or total ERK1/2 (1:1000 dilution; Cell Signaling Technology), rabbit monoclonal antibody against phosphorylated Akt (1:2000 dilution; Cell Signaling Technology), rabbit polyclonal antibody against phosphorylated mTOR (mammalian target of rapamycin; 1:1000 dilution; Cell Signaling Technology), rabbit polyclonal antibody against TGFβR2 (TGFβ receptor 2; 1:500 dilution; Millipore), mouse monoclonal antibody against phosphorylated tyrosine (1:2000 dilution; Cell Signaling Technology), or mouse monoclonal antibody against GAPDH (glyceraldehyde-3-phosphate dehydrogenase; 1:10000 dilution; KangChen Bio-Tech). .. After extensive washing in TBST (TBS with 0.03% Tween 20), the membrane was incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:10000 dilution; KangChen Bio-Tech) for 2 h at room temperature.

    Article Title: Neuritin Activates Insulin Receptor Pathway to Up-regulate Kv4.2-mediated Transient Outward K + Current in Rat Cerebellar Granule Neurons
    Article Snippet: The proteins were separated on a 10% SDS-polyacrylamide gel, transferred to polyvinylidene difluoride membranes (Millipore). .. The membrane was blocked with 10% nonfat milk and incubated at 4 °C overnight with mouse monoclonal antibody against Kv4.2, Kv4.3, Kv1.1 (1:2000, catalogue numbers 75-016, 75-017, and 75-007; University of California, Davis), or rabbit polyclonal antibody against the phosphorylated insulin receptor β subunit (1:1000, catalogue number 3024; Cell Signaling Technology), or rabbit monoclonal antibody against phosphorylated ERK1/2 or total ERK1/2 (1:1000, catalogue numbers 4370 and 4695; Cell Signaling Technology), or rabbit monoclonal antibody against phosphorylated Akt (1:2000, catalogue number 4060; Cell Signaling Technology), or rabbit polyclonal antibody against phosphorylated mTOR (1:1000, catalogue number 2971; Cell Signaling Technology), or rabbit polyclonal antibody against phosphorylated IRS-1 (1:500, catalogue number 3070; Cell Signaling Technology), or mouse monoclonal antibody against GAPDH (1:10,000, catalogue number KC-5G4; KangChen Bio-Tech, China). .. After extensively washing in TBST, the membrane was incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:10,000, catalogue numbers KC-MM-035 and KC-RB-035; KangChen Bio-Tech) for 2 h at room temperature.



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    Cell Signaling Technology Inc rabbit polyclonal antibody against phosphorylated insulin receptor β subunit
    Rabbit Polyclonal Antibody Against Phosphorylated Insulin Receptor β Subunit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+phosphorylated+insulin+receptor+%CE%B2+subunit/rabbit+polyclonal+antibody+against+phosphorylated+insulin+receptor+%CE%B2+subunit/10__1042_slash_bj20140155-59-37-48
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody against phosphorylated insulin receptor β subunit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc rabbit polyclonal antibody against the phosphorylated insulin receptor β subunit
    Rabbit Polyclonal Antibody Against The Phosphorylated Insulin Receptor β Subunit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+phosphorylated+insulin+receptor+%CE%B2+subunit/rabbit+polyclonal+antibody+against+phosphorylated+insulin+receptor+%CE%B2+subunit/pmc03510849-74-7-21
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody against the phosphorylated insulin receptor β subunit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

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